Apex® Biological Indicators
-
Apex® Biological Indicators
Apex® Biological Indicator (BI) products are designed specifically to meet the unique needs of the pharmaceutical, food, and medical device industries utilizing vaporized hydrogen peroxide (VH2O2) decontamination.
Our Apex® product line offers:
- The most resistant strain to VH202 process to assure the manufacturing area is decontaminated
- Greatly reduced false positives because products match the decontamination process
- A highly flexible portfolio of BI’s to match the resistance of process parameters
Whether it be stainless steel discs/ribbons or a spore suspension, Apex® BI products offer the ideal solution to monitoring hydrogen peroxide decontamination, utilizing vaporized hydrogen peroxide (VH202) decontamination.

Apex® Discs
-
Inoculated onto grade 304 stainless steel carriers
-
Discs measure 0.35″ diameter x 0.008″ thick
-
Available with G. sterothermophilus 12980 or 7953, or B. atorphaeus 9372
-
Tyvek® packaged indicators have perforations for hanging and a thumb notch for peeling

Apex® Ribbons
- Bare stainless steel ribbons measure (0.25″ x 2.75″)
- Inoculated at one end
- Available with G. sterothermophilus 12980
- Convenient size and flexibility

Tri-Scale Biological Indicator
- One convenient Tyvek/Tyvek® package; three G. stearothermophilus 12980 populations
- Three carriers with 104, 105 and 106 spores; sealed in separate compartments
- Used for initial shakedown or validation of new enclosures or filling lines
- Convenient to identify worst-case location(s) in enclosure
Technical Specifications
Apex® Vaporized Hydrogen Peroxide Biological Indicators
Storage: 2 – 8°C; < 50% relative humidity
Expiration: 9 months from date of manufacture (not applicable for Apex BIs with 103 populations)
Certification: Apex biological indicators are traceable to a recognized culture collection, and certified for population and reference D-value.
G. stearothermophilus 12980
Incubation | 7 days at 55 – 60°C | |||||
Population | 106 | 103 | 10⁴ | 106 |
104 105 106 |
106 |
Units | 100 | 100 | 100 | 100 | 50 | 100 |
Reorder# |
HMV-091 |
HMV-091E3 |
KCD-404 |
SBC-327 |
LOG-456 |
NAS-152 |
B. atrophaeus
Incubation |
7 days at 30 – 35°C |
7 days at 30 – 35°C |
Population | 106 | 103 |
Units | 100 | 100 |
Reorder# |
GRS-090 |
GRS-090E3 |
Document Downloads
- Product Brochure – Vaporized Hydrogen Peroxide Biological Indicators
- Product Specification – Apex Spore Suspension Geobacillus stearothermophilus 7953 or 12980
- Product Specifications – Apex Discs Geobacillus stearothermophilus 7953
- Product Specifications – Apex Discs Geobacillus stearothermophilus 12980
- Product Specifications – Apex Ribbon Geobacillus stearothermophilus 12980
- Product Specifications -Apex Discs Bacillus atrophaeus 9372
- Product Specifications – Apex Disc Tri-Scale Geobacillus stearothermophilus 12980
- Technical Report – Apex
- Population Assay Instructions – Apex Products
- Safety Data Sheet – Apex
- Spore News – Proper BI Placement During VHP Decontamination Cycles
- Spore News: Using Replicate BIs to Evaluate Bio-decontamination
- Spore News – Parameters Effecting Vapor Hydrogen Peroxide Biological Indicator Performance
Related products
Related services

Contract studies laboratory
Mesa’s independent contract studies laboratory performs certified and compliant third-party chemical and biological indicator testing and verification.
Learn more

Sterilization cycle development
Get expert help for your bioburden. Mesa Labs assists with sterilization cycle development, BI qualification and validation, and routine monitoring support.
Learn more