Apex® Biological Indicators
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Apex® Biological Indicators
Apex Biological Indicator (BI) products are designed specifically to meet the unique needs of the pharmaceutical, food, and medical device industries utilizing vaporized hydrogen peroxide (VH2O2) decontamination.
Our Apex product line offers:
- The most resistant strain to VH202 processes to assure the manufacturing area is decontaminated
- Greatly reduced false positives because products match the decontamination process
- A highly flexible portfolio of BIs to match the resistance of process parameters
Whether it be self-contained biological indicators, stainless steel discs/ribbons or a spore suspension, Apex BI products offer the ideal solution for validating and monitoring vaporized hydrogen peroxide (VH202) decontamination.

Apex EZTest
- Self-contained Biological Indicator
- Available with G. stearothermophilus 12980
- Easy to culture - no laboratory required
- Dramatic Color Change

Apex Discs
- Inoculated onto grade 304 stainless steel carriers
- Discs measure 0.35″ diameter x 0.008″ thick
- Available with G. stearothermophilus 12980 or 7953, or B. atrophaeus 9372
- Tyvek® packaged indicators have perforations for hanging and a thumb notch for peeling

Apex Ribbons
- Bare stainless steel ribbons measure (0.25″ x 2.75″)
- Inoculated at one end
- Available with G. stearothermophilus 12980
- Convenient size and flexibility

Tri-Scale Biological Indicator
- One convenient Tyvek/Tyvek® package; three G. stearothermophilus 12980 populations
- Three carriers with 104, 105 and 106 spores; sealed in separate compartments
- Used for initial shakedown or validation of new enclosures or filling lines
- Convenient to identify worst-case location(s) in enclosure
Technical Specifications
Apex® Vaporized Hydrogen Peroxide Biological Indicators
Storage: 2 – 8°C; < 50% relative humidity
Expiration: 9 months from date of manufacture (not applicable for Apex BIs that are made to order)
Certification: Apex biological indicators are traceable to a recognized culture collection, and certified for population and reference D-value.
G. stearothermophilus 12980
Incubation |
7 days at 55 – 60°C |
|||||
Population | 106 | 10⁴ | 103 | 106 |
104 105 106 |
106 |
Units |
100 |
100 |
100 |
100 |
50 |
30 |
Reorder# |
HMV-091 |
KCD-404 |
HMV-091E3* |
SBC-327 |
LOG-456 |
AP-VH2O2-SC-S12980/6 |
*product is made to order
G. stearothermophilus 7953
Incubation | 7 days at 55 – 60°C | ||
Population | 106 | 104 | 103 |
Units | 100 | 100 | 100 |
Reorder# |
NAS-152 |
NAS-152E4* |
NAS-152E3* |
*product is made to order
B. atrophaeus 9372
Incubation |
7 days at 30 – 35°C
|
||
Population | 106 | 105 | 103 |
Units | 100 | 100 | 100 |
Reorder# |
GRS-090 |
GRS-090E5* |
GRS-090E3* |
*product is made to order
Document Downloads
- Product Brochure – Vaporized Hydrogen Peroxide Biological Indicators
- Product Specification - Apex EZTest
- Product Specification – Apex Spore Suspension Geobacillus stearothermophilus 7953 or 12980
- Product Specifications – Apex Discs Geobacillus stearothermophilus 7953
- Product Specifications – Apex Discs Geobacillus stearothermophilus 12980
- Product Specifications – Apex Ribbon Geobacillus stearothermophilus 12980
- Product Specifications -Apex Discs Bacillus atrophaeus 9372
- Product Specifications – Apex Disc Tri-Scale Geobacillus stearothermophilus 12980
- Technical Report – Apex
- Technical Report - Apex EZTest
- Population Assay Instructions – Apex Products
- IFU - Apex EZTest Activation Instructions
- Safety Data Sheet – Apex
- Safety Data Sheet - Apex EZTest
- Spore News – Proper BI Placement During VHP Decontamination Cycles
- Spore News - Using Replicate BIs to Evaluate Bio-decontamination
- Spore News – Parameters Effecting Vapor Hydrogen Peroxide Biological Indicator Performance
- Spore News - SEM analysis of VPHP Biological Indicators
- Spore News - Vaporized Hydrogen Peroxide Isolator Decontamination in a World of Uncertainty: A Modern-Day Field Guide
- Spore News - Biological Indicators for Vaporized Hydrogen Peroxide Isolator Decontamination: Characteristics, Uses and Challenges
- Video - Apex EZTest Activation
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