Apex® Biological Indicators
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Apex Biological Indicators
Validating and Monitoring Vaporized Hydrogen Peroxide (VH2O2) Decontamination.
Designed exclusively for the pharmaceutical, food, and medical device industries, Apex products are tailor-made to meet your unique needs.
Why choose Apex Biological Indicators? Here's what we offer:
Unmatched Resistance: Our products feature the most resilient strain to VH2O2 processes, ensuring thorough decontamination of your manufacturing area.
Minimized False Positives: Say goodbye to unnecessary false positives! Apex biological indicator products are carefully designed to closely match the decontamination process, greatly reducing false positives.
Flexibility at its Finest: With our highly versatile portfolio, you can easily find the perfect match for your specific process parameters.
Whether you prefer self-contained biological indicators, packaged discs, unpackaged ribbons, or spore suspensions, Apex products offer the ideal solution for validating and monitoring VH2O2 decontamination.
Download the Product Overview of Apex Biological Indicators

Apex EZTest®
- Self-contained Biological Indicator
- Available with G. stearothermophilus 12980
- Easy to culture - no laboratory required
- Dramatic Color Change

Apex Discs
- Inoculated on grade 304 stainless steel carriers.
- Size: 0.35″ diameter x 0.008″ thick.
- Options: G. stearothermophilus 12980 or 7953, or B. atrophaeus 9372.
- Tyvek® packaged indicators with hanging perforations and thumb notch.

Apex Ribbons
- Bare stainless steel ribbons measure (0.25″ x 2.75″)
- Inoculated at one end
- Available with G. stearothermophilus 12980
- Convenient size and flexibility

Tri-Scale Biological Indicator
- One Tyvek package; three G. stearothermophilus 12980 populations.
- Three carriers: 104, 105, and 106 spores; sealed separately.
- For initial shakedown or validation of enclosures/filling lines.
- Convenient to identify worst-case location(s) in enclosure
Technical Specifications
Apex® Vaporized Hydrogen Peroxide Biological Indicators
Storage: 2 – 8°C; < 50% relative humidity
Expiration: 9 months from date of manufacture (not applicable for Apex BIs with 103 populations)
Certification: Apex biological indicators are traceable to a recognized culture collection, and certified for population and reference D-value.
G. stearothermophilus 12980
| Incubation | 7 days at 55 – 60°C | |||||
| Population | 106 | 103 | 10⁴ | 106 |
104 105 106 |
106 |
| Units | 100 | 100 | 100 | 100 | 50 | 100 |
| Reorder# |
HMV-091 |
HMV-091E3 |
KCD-404 |
SBC-327 |
LOG-456 |
NAS-152 |
B. atrophaeus
| Incubation |
7 days at 30 – 35°C |
7 days at 30 – 35°C |
| Population | 106 | 103 |
| Units | 100 | 100 |
| Reorder# |
GRS-090 |
GRS-090E3 |
Document Downloads
- Product Brochure – Vaporized Hydrogen Peroxide Biological Indicators
- Product Specification – Apex Spore Suspension Geobacillus stearothermophilus 7953 or 12980
- Product Specifications – Apex Discs Geobacillus stearothermophilus 7953
- Product Specifications – Apex Discs Geobacillus stearothermophilus 12980
- Product Specifications – Apex Ribbon Geobacillus stearothermophilus 12980
- Product Specifications -Apex Discs Bacillus atrophaeus 9372
- Product Specifications – Apex Disc Tri-Scale Geobacillus stearothermophilus 12980
- Technical Report – Apex
- Population Assay Instructions – Apex Products
- Safety Data Sheet – Apex
- Spore News – Proper BI Placement During VHP Decontamination Cycles
- Spore News: Using Replicate BIs to Evaluate Bio-decontamination
- Spore News – Parameters Effecting Vapor Hydrogen Peroxide Biological Indicator Performance
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